Effects of various carbohydrate substrates on yeast fermentation

Get Full Essay Get access to this section to get all help you need with your essay and educational issues. Under anaerobic conditions when Oxygen is unavailable yeast cells will break down carbohydrate substrates into ethanol and Carbon Dioxide. This is known as fermentation and is explained using the following equation:

Effects of various carbohydrate substrates on yeast fermentation

Cellular respiration and alcohol fermentation are examples of this characteristic. Yeast, a single celled organism conducts fermentation when sugar is present, to make chemical energy, and in the process produce alcohol and carbon dioxide.

Experiments were performed to understand this process better. One experiment focused on yeast and glucose, while another observed how yeast fermented and produced CO2 with different types of sugars.

Respirometers were used to record the amount of CO2 production. Both experiments proved to be informative, with all expected sugars fermenting the yeast and producing carbon dioxide.

However, the exact predictions made were not fully supported by the results and when comparing to the results of a different group, questions are brought to the surface on the performance the experiments. Should the experiment be administered again with minor changes to protocol?

Introduction Cellular respiration is a process that most living organisms undergo to create and obtain chemical energy in the form of adenosine triphosphate ATP.

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The energy is synthesized in three separate stages of cellular respiration: Glycolysis and the citric acid cycle are both anaerobic pathways because they do not need oxygen to form energy.

The electron transport chain however, is aerobic due to its use of oxidative phosphorylation.

Effects of various carbohydrate substrates on yeast fermentation

Oxidative phosphorylation is the process in which ATP molecules are produced with the assistance of oxygen molecules. Campbell et al Fermentation is a process adopted, typically, by anaerobic organisms to obtain ATP without the use of oxygen.

ABSTRACT The effect of the nature of substrate on the rate of respiration of yeast was determined using the Smith fermentation tube method. 15 mL of both distilled water (H2O) and 10% yeast solution was poured in six fermentation tubes. ABSTRACT The effect of the nature of substrate on the rate of respiration of yeast was determined using the Smith fermentation tube method. 15 mL of both distilled water (H2O) and 10% yeast solution was poured in six fermentation tubes. Fermentation Formal Lab Report. The purpose of this group’s research was to see the effects of yeast fermentation with glucose, sucrose, fructose and lactose. 25 Time (min) Figure 4 The production of CO2, through fermentation of yeast and various sugars, over a period of time. Experiment B. Figure 4 demonstrates the amount of CO2.

Organisms that have this ability are called facultative anaerobes. When yeast is in the presence of oxygen it performs cellular respiration, but when oxygen is absent it undergoes alcohol fermentation. In alcohol fermentation, the sugar is converted into two 3-Carbon sugars known as pyruvate.

The pyruvate is then converted into ethanol alcohol in two steps. In the first step, it is converted into acetaldehyde with 2 the release of carbon dioxide CO2the independent variable of the experiment. Next the acetaldehyde is reduced to ethanol. Campbell et al The experiments performed in Dr.

Two experiments were performed a week apart. The first experiment, experiment A, studied the fermentation exclusively between yeast and glucose. A respirometer was used to observe the amount of CO2 produced during fermentation. Based on previous knowledge of fermentation activity, it can be concluded that a test tube containing a greater volume of yeast, rather than glucose, would have the most CO2 production, because fermentation of glucose is dependent on yeast.

The second experiment, experiment B, was designed by the students, with each lab group making their own design. The structures of each of the sugars suggests that the test tube containing glucose would ferment the most because it would require less work to break down into pyruvate and acetaldehyde Fig.

The yeast, because of its lack of appropriate enzymes, would not ferment lactose. Figure 2 Molecular structure of lactose.

Photo courtesy of http: The resipormeters used in the experiment consisted of a 1 mL serological pipette, aquarium tubing, binder clip, and a test tube. First, the aquarium tubing was attached to the pipette, which was then placed in a beaker filled half way with water.

A pipette pump was attached to the tubing and then drew water from the beaker, up to the 0 mL mark. With the pipette pump still attached, the tubing was bent and the clip placed at the bend, as a way to prevent the liquid from falling back into the tube. Next the test tubes for the experiment were prepared.

The test tubes were labeled 1, 2, 3, and 4. Necessary amounts of water and glucose were added to all test tubes Table 1. The yeast was added last to avoid reaction occurring sooner than needed. As soon as the five minutes had passed, the respirometers were added to each test tube and made ready for obtaining data.

Immediately after they were added, the timer started. The liquid level was recorded every two minutes for 20 minutes.Type Of Sugar on Yeast Fermentation Lab Purpose: To measure and analyze the effects of various types and masses of sugar in an ethanol fermentation reaction with yeast.

Introduction: Ethanol fermentation is a system in which hydrogen ions from NADH + H + are broken down in order to release the trapped energy and regenerate NAD+.

Type Of Sugar on Yeast Fermentation Lab Purpose: To measure and analyze the effects of various types and masses of sugar in an ethanol fermentation reaction with yeast. Introduction: Ethanol fermentation is a system in which hydrogen ions from NADH + H + are broken down in order to release the trapped energy and regenerate NAD+.

Effects of Various Carbohydrate Substrates on Yeast Fermentation Abstract This experiment was performed to determine which carbohydrate substrates positively influence yeast fermentation since yeast seems to have greater ability to . Published: Mon, 5 Dec The aim of this investigation is to examine what effects different substrates have on the respiration of yeast.

I will investigate this by measuring the amount of carbon dioxide evolved during anaerobic respiration. the Effects of Mass and Type Of Sugar on Yeast Fermentation Lab Purpose: To measure and analyze the effects of various types and masses of sugar in an ethanol fermentation reaction with yeast.

Introduction: Ethanol fermentation is a system in which hydrogen ions from NADH + H + are broken down in order to release the trapped energy and. The suitability of delignified cellulosic (DC) material supported kefir yeast to ferment raw materials that contain various single carbohydrates, for the production of potable alcohol and alcoholic drinks, is examined in this investigation.

Yeast And Different Carbohydrate Substrates | Essay Example